Biochem J. 1978 Oct 1;175(1):349-52.

The immobilization of adenine nucleotides on polysaccharides by using glutaraldehyde coupling and borohydride reduction.

Abstract

Adenine nucleotides were immobilized on modified Sepharose 4B or Dextran T40 with glutaraldehyde and reduced with KBH4. Binding was dependent on pH and the nature of the amino group on the modified polysaccharide. ATP bound to soluble dextran retained coenzyme activity with glycerol kinase. Binding is proposed to occur via a Schiff base.

PMID:: 104714; [PubMed - indexed for MEDLINE]
PMCID:: PMC1186073

Free PMC Article

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

GLUTARALDEHYDE - HSDB

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Comparative studies on the properties of tryptophanase and tyrosine phenol-lyase immobilized directly on Sepharose or by use of Sepharose-bound pyridoxal 5'-phosphate. [Eur J Biochem. 1975]
Comparative studies on the properties of tryptophanase and tyrosine phenol-lyase immobilized directly on Sepharose or by use of Sepharose-bound pyridoxal 5'-phosphate.
Fukui S, Ikeda S, Fujimura M, Yamada H, Kumagai H. Eur J Biochem. 1975 Feb 3; 51(1):155-64.
The preparation of stable enzyme-coenzyme complexes with endogenous catalytic activity. [Biochem J. 1979]
The preparation of stable enzyme-coenzyme complexes with endogenous catalytic activity.
Gacesa P, Venn RF. Biochem J. 1979 Jan 1; 177(1):369-72.
An essential role for constitutive Schiff base-forming ligands in antigen presentation to murine T cell clones. [J Immunol. 1990]
An essential role for constitutive Schiff base-forming ligands in antigen presentation to murine T cell clones.
Gao XM, Rhodes J. J Immunol. 1990 Apr 15; 144(8):2883-90.
The interaction of beta-cyclodextrin with dinucleoside phosphates. [Biopolymers. 1974]
The interaction of beta-cyclodextrin with dinucleoside phosphates.
Formoso C. Biopolymers. 1974 May; 13(5):909-17.
Review The taxonomy of binding sites in proteins. [Mol Cell Biochem. 1978]
Review The taxonomy of binding sites in proteins.
Rossmann MG, Argos P. Mol Cell Biochem. 1978 Nov 16; 21(3):161-82.

See reviews... See all...

Cited by 3 PubMed Central articles

In vitro nuclear interactome of the HIV-1 Tat protein. [Retrovirology. 2009]
In vitro nuclear interactome of the HIV-1 Tat protein.
Gautier VW, Gu L, O'Donoghue N, Pennington S, Sheehy N, Hall WW. Retrovirology. 2009 May 19; 6:47. Epub 2009 May 19.
Selective glutaraldehyde-mediated coupling of proteins to the 3'-adenine terminus of polymerase chain reaction products. [J Biomol Tech. 2008]
Selective glutaraldehyde-mediated coupling of proteins to the 3'-adenine terminus of polymerase chain reaction products.
Bruno JG, Crowell R. J Biomol Tech. 2008 Jul; 19(3):177-83.
The preparation of stable enzyme-coenzyme complexes with endogenous catalytic activity. [Biochem J. 1979]
The preparation of stable enzyme-coenzyme complexes with endogenous catalytic activity.
Gacesa P, Venn RF. Biochem J. 1979 Jan 1; 177(1):369-72.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound
PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance
PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

The immobilization of adenine nucleotides on polysaccharides by using glutaralde...
The immobilization of adenine nucleotides on polysaccharides by using glutaraldehyde coupling and borohydride reduction.
Biochem J. 1978 Oct 1 ;175(1):349-52.

PubMed
HIV-1 Nef mediates lymphocyte chemotaxis and activation by infected macrophages.
HIV-1 Nef mediates lymphocyte chemotaxis and activation by infected macrophages.
Nat Med. 1999 Sep ;5(9):997-103.

PubMed
[Level of nicotinamide coenzymes in the liver and myocardium of rats poisoned wi...
[Level of nicotinamide coenzymes in the liver and myocardium of rats poisoned with dichlorethane].
Biull Eksp Biol Med. 1975 Apr ;79(4):58-60.

PubMed
High frequency of adhesion defects in B-lineage acute lymphoblastic leukemia.
High frequency of adhesion defects in B-lineage acute lymphoblastic leukemia.
Blood. 1999 Jul 15 ;94(2):754-64.

PubMed
Nonvitellogenic female sterile mutants and the regulation of vitellogenesis in D...
Nonvitellogenic female sterile mutants and the regulation of vitellogenesis in Drosophila melanogaster.
Dev Biol. 1978 Nov ;67(1):202-13.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: