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1: J Gen Virol. 1998 Aug;79 ( Pt 8):1895-9.Click here to read Links

Mutation in NS5 protein attenuates mouse neurovirulence of yellow fever 17D vaccine virus.

Xie H, Ryman KD, Campbell GA, Barrett AD.

Center for Tropical Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston 77555-0605, USA.

The 17D-204 vaccine manufactured in South Africa (17D-204-SA) and a large plaque variant (17D-LP) derived from it were highly virulent in adult mice. The LD50 of 17D-LP virus was 0-2 p.f.u. for mice following intracerebral inoculation. In comparison, a medium plaque variant derived from 17D-LP, termed 17D-MP virus, was found to be attenuated in adult mice following the same route of inoculation (> 10(4) p.f.u./LD50). Replication of 17D-MP virus was decreased in infected mouse brains compared to 17D-LP virus. Also, 17D-MP virus was slightly temperature sensitive at 39.5 degrees C. Compared to its parent viruses, 17D-204-SA and 17D-LP, 17D-MP virus had one unique mutation at nt 8045 in the genome which resulted in a single amino acid substitution (Pro --> Ser) at residue 137 of the NS5 protein and appeared to be the mutation responsible for the attenuation of 17D-MP virus. This is the first time that altered virulence of a flavivirus caused by mutation in a non-structural protein gene, other than NS1, has been reported.

PMID: 9714237 [PubMed - indexed for MEDLINE]

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    Yellow fever is a serious disease caused by the yellow fever virus. It is spread through the bite of an infected mosquito andcannot be spread directly from person to person. It is found in certain parts of Africa and Sou...

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