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1: Cell. 1998 Jul 24;94(2):205-16.Click here to read Links

PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization.

Wan L, Molloy SS, Thomas L, Liu G, Xiang Y, Rybak SL, Thomas G.

Vollum Institute, The Oregon Health Sciences University, Portland 97201, USA.

We report the role of one member of a novel gene family, PACS-1, in the localization of trans-Golgi network (TGN) membrane proteins. PACS-1 directs the TGN localization of furin by binding to the protease's phosphorylated cytosolic domain. Antisense studies show TGN localization of furin and mannose-6-phosphate receptor, but not TGN46, is strictly dependent on PACS-1. Analyses in vitro and in vivo show PACS-1 has properties of a coat protein and connects furin to components of the clathrin-sorting machinery. Cell-free assays indicate TGN localization of furin is directed by a PACS-1-mediated retrieval step. Together, these findings explain a mechanism by which membrane proteins in mammalian cells are localized to the TGN.

PMID: 9695949 [PubMed - indexed for MEDLINE]