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1: Genomics. 1997 Jun 1;42(2):295-301.Click here to read Links

Transcript mapping in a 46-kb sequenced region at the core of 12q13.3 amplification in human cancers.

Elkahloun AG, Krizman DB, Wang Z, Hofmann TA, Roe B, Meltzer PS.

Laboratory of Cancer Genetics, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892-4470, USA.

We used a combination of sequence analysis and exon trapping in an effort to determine the complete transcript map for a cosmid (6E5) derived from 12q13.3, a region of DNA sequence amplification in human cancers. This cosmid, previously known to contain three genes (CDK4, SAS, and OS9), was sequenced, and that information was used for computer-assisted analysis. In addition, 6E5 was subjected to both internal and 3'-terminal exon-trapping protocols, and the results of these studies were used to guide cDNA cloning experiments. These studies demonstrate that this cosmid is derived from a remarkably gene-dense region and add two new transcripts (KIAA0167 and 6E5.2) to the list of sequences that are expressed in tumors bearing amplification of this region.

PMID: 9192850 [PubMed - indexed for MEDLINE]