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1: Microbiology. 1997 Feb;143 ( Pt 2):377-86.Click here to read Links

The topoisomerase I gene from Candida albicans.

Jiang W, Gerhold D, Kmiec EB, Hauser M, Becker JM, Koltin Y.

ChemGenics Pharmaceuticals Inc., Cambridge, MA 02139, USA.

We report here the cloning of the Candida albicans genomic topoisomerase I gene (TOP1) by use of PCR and subsequent hybridization. The predicted protein sequence shared 58.8% identity with the Saccharomyces cerevisiae topoisomerase I and 30-50% identity with other eukaryotic topoisomerase I proteins. A conditional gene disruption strain (CWJ477) was constructed so that one copy of TOP1 was deleted and the other copy of TOP1 was placed under a regulatable promoter. Under repressed conditions, cells grew slowly and cell morphology was abnormal. The virulence of CWJ477 was markedly reduced in a mouse model system, and that of the single gene knockout-strain was slightly attenuated, indicating that TOP1 might play a role in the infection of C. albicans in mice in a dose-dependent manner. Despite the reduced virulence of both the single and double knockout strains, viable cells of the pathogen were recovered from the kidneys as late as 22 d post-infection.

PMID: 9043115 [PubMed - indexed for MEDLINE]