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1: FEBS Lett. 1997 Feb 3;402(2-3):162-6.Click here to read Links

Murine epidermal lipoxygenase (Aloxe) encodes a 12-lipoxygenase isoform.

Kinzig A, Fürstenberger G, Bürger F, Vogel S, Müller-Decker K, Mincheva A, Lichter P, Marks F, Krieg P.

Research Program on Tumor Cell Regulation, Deutsches Krebsforschungszentrum, Heidelberg, Germany.

Using a combination of conventional screening procedures and polymerase chain reaction cloning, we have isolated a cDNA encoding an epidermis-type 12-lipoxygenase (e12-lipoxygenase) from mouse epidermis. The open reading frame corresponds to a protein of 662 amino acids and was found to be 99.8% identical to the ORF of an epidermal lipoxygenase gene Aloxe, described recently [Van Dijk et al. (1995) Biochim. Biophys. Acta 1259, 4-8]. When expressed in human embryonic kidney cells the recombinant protein could be shown to synthesize 12(S)-HETE from arachidonic acid. By fluorescence in situ hybridization the e12-lipoxygenase gene was localized to chromosome band 11 B1-B3.

PMID: 9037187 [PubMed - indexed for MEDLINE]