Characteristic sequences in the promoter region of the chicken tyrosinase-encoding gene.

Department of Anatomy and Cell Biology, University of Cape Town Medical School, Observatory, South Africa.

We have isolated and sequenced a genomic DNA sequence encoding chicken tyrosinase (TYR) that includes 2125 nt of 5' flanking sequence, the first exon and a part of the first intron. The 5' flanking sequence was able to drive transcription of a reporter gene in immortalised quail neural crest cells. The sequence, which is the most extensive to be reported for a lower vertebrate TYR gene to date, was further analyzed using primer extension and computer-aided homology searches. Transcription initiation appears to occur at heterogeneous start points and in the absence of a TATA box, but may be mediated via a potential initiator (Inr) element and Sp1-binding motif. We have identified two evolutionarily conserved regions within the 5' flanking sequence that may be functionally significant, as they contain regulatory elements previously reported to play a role in melanocyte-specific expression of TYR in mammals. This study contributes towards an understanding of the requirements for melanocyte-specific TYR expression in lower vertebrates.

PMID: 8647445 [PubMed - indexed for MEDLINE]