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1: Gene. 1996 Feb 22;169(1):135-6.Click here to read Links

Sequence of the Bacillus stearothermophilus gene encoding aspartokinase II.

Cantoni R, Labó M, De Rossi E, Riccardi G.

Dipartimento di Genetica e Microbiologia, University of Pavia, Italy.

The complete nucleotide sequence of the gene encoding aspartokinase (Ask) II from thermophilic Bacillus stearothermophilus has been determined. Degenerate oligodeoxyribonucleotides primed the amplification of a 932-bp gene. This sequence was successively used for constructing new primers applied in inverse polymerase chain reaction using, as template, self-ligating DNA fragments. The deduced amino-acid sequence is 68.7% identical with the sequence of the Bacillus sp. strain MGA3 Ask II.

PMID: 8635739 [PubMed - indexed for MEDLINE]