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The amino-acid sequence similarity of plant glutamate dehydrogenase to the extremophilic archaeal enzyme conforms to its stress-related function.
Department of Biology, University of Crete, Heraklion, Greece.
A cDNA clone encoding grapevine (Vitis vinifera L. cv Sultanina) NAD(H)-glutamate dehydrogenase (GDH) was isolated from a cDNA expression library by immunoscreening with a polyclonal antibody raised against grapevine GDH. Nucleotide sequence analysis revealed an open reading frame (ORF) encoding a precursor protein of 411 amino acids (aa) with a calculated molecular mass of 44.517 kDa. The deduced aa sequence showed relatively higher homology to GDH from archaebacteria species, than to those from eukaryotes and eubacteria. This resemblance indicated a functional and/or evolutionary relationship in this class of enzymes which might be relevant to the stress-related function of plant GDH. We have shown that the bacterially produced plant GDH was thermostable.
PMID: 8626071 [PubMed - indexed for MEDLINE]
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Cited by 3 PubMed Central articles
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The isoenzyme 7 of tobacco NAD(H)-dependent glutamate dehydrogenase exhibits high deaminating and low aminating activities in vivo.
Skopelitis DS, Paranychianakis NV, Kouvarakis A, Spyros A, Stephanou EG, Roubelakis-Angelakis KA.
Plant Physiol. 2007 Dec; 145(4):1726-34. Epub 2007 Oct 11.
[Plant Physiol. 2007]
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Abiotic stress generates ROS that signal expression of anionic glutamate dehydrogenases to form glutamate for proline synthesis in tobacco and grapevine.
Skopelitis DS, Paranychianakis NV, Paschalidis KA, Pliakonis ED, Delis ID, Yakoumakis DI, Kouvarakis A, Papadakis AK, Stephanou EG, Roubelakis-Angelakis KA.
Plant Cell. 2006 Oct; 18(10):2767-81. Epub 2006 Oct 13.
[Plant Cell. 2006]
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Characterization and expression of NAD(H)-dependent glutamate dehydrogenase genes in Arabidopsis.
Turano FJ, Thakkar SS, Fang T, Weisemann JM.
Plant Physiol. 1997 Apr; 113(4):1329-41.
[Plant Physiol. 1997]