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A DEAD-box RNA helicase in the Escherichia coli RNA degradosome.
Nuffield Department of Clinical Biochemisstry and Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, UK.
The Escherichia coli RNA degradosome is a multi-enzyme complex that contains the exoribonuclease polynucleotide phosphorylase (PNPase) and the endoribonuclease RNase E. Both enzymes are important in RNA processing and messenger RNA degradation. Here we report that enolase and RhlB are two other major components of the degradosome. Enolase is a glycolytic enzyme with an unknown role in RNA metabolism. RhlB is a member of the DEAD-box family of ATP-dependent RNA helicases, which are found in both prokaryotes and eukaryotes. We show that the degradosome has an ATP-dependent activity that aids the degradation of structured RNA by PNPase. Incubation of the degradosome with affinity-purified antibody against RhlB inhibited the ATP-stimulated RNA degradation. These results suggest that RhlB acts by unwinding RNA structures that impede the processive activity of PNPase. RhlB is thus an important enzyme in mRNA turnover.
PMID: 8610017 [PubMed - indexed for MEDLINE]
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Cited by over 100 PubMed Central articles
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Regulation of ribonuclease E activity by the L4 ribosomal protein of Escherichia coli.
Singh D, Chang SJ, Lin PH, Averina OV, Kaberdin VR, Lin-Chao S.
Proc Natl Acad Sci U S A. 2009 Jan 20; 106(3):864-9. Epub 2009 Jan 14.
[Proc Natl Acad Sci U S A. 2009]
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Loss of RNase R induces competence development in Legionella pneumophila.
Charpentier X, Faucher SP, Kalachikov S, Shuman HA.
J Bacteriol. 2008 Dec; 190(24):8126-36. Epub 2008 Oct 10.
[J Bacteriol. 2008]
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Polynucleotide phosphorylase hinders mRNA degradation upon ribosomal protein S1 overexpression in Escherichia coli.
Briani F, Curti S, Rossi F, Carzaniga T, Mauri P, Dehò G.
RNA. 2008 Nov; 14(11):2417-29. Epub 2008 Sep 29.
[RNA. 2008]
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