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Identification and characterization of CPP32/Mch2 homolog 1, a novel cysteine protease similar to CPP32.
Vertex Pharmaceuticals Incorporated, Cambridge, Massachusetts 02139, USA.
We have identified and characterized a novel cysteine protease named CMH-1 that is a new member of the interleukin 1 beta converting enzyme (ICE) family of proteases with substrate specificity for Asp-X. CMH-1 has the highest similarity to CPP32 (52% amino acid identity) and MCH2 (31% identical). CMH-1 shares conserved amino acid residues that form the core structure of ICE as well as those residues involved in catalysis and in the P1 aspartate binding. Overexpression of CMH-1 in COS cells resulted in the processing of CMH-1 and the induction of apoptosis of transfected cells. Coexpression of CMH-1 with poly(ADP-ribose) polymerase (PARP) also resulted in a specific cleavage of PARP. Purified recombinant CMH-1 cleaved PARP but not interleukin 1 beta precursor in vitro.
PMID: 8567622 [PubMed - indexed for MEDLINE]
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Cited by 15 PubMed Central articles
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Differential regulation of human YY1 and caspase 7 promoters by prohibitin through E2F1 and p53 binding sites.
Joshi B, Rastogi S, Morris M, Carastro LM, DeCook C, Seto E, Chellappan SP.
Biochem J. 2007 Jan 1; 401(1):155-66.
[Biochem J. 2007]
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Caspase activation and neuroprotection in caspase-3- deficient mice after in vivo cerebral ischemia and in vitro oxygen glucose deprivation.
Le DA, Wu Y, Huang Z, Matsushita K, Plesnila N, Augustinack JC, Hyman BT, Yuan J, Kuida K, Flavell RA, et al.
Proc Natl Acad Sci U S A. 2002 Nov 12; 99(23):15188-93. Epub 2002 Nov 1.
[Proc Natl Acad Sci U S A. 2002]
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Caspase-3 controls both cytoplasmic and nuclear events associated with Fas-mediated apoptosis in vivo.
Zheng TS, Schlosser SF, Dao T, Hingorani R, Crispe IN, Boyer JL, Flavell RA.
Proc Natl Acad Sci U S A. 1998 Nov 10; 95(23):13618-23.
[Proc Natl Acad Sci U S A. 1998]
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