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1: Genomics. 1995 Jul 20;28(2):163-70.Click here to read Links

RNA expression and chromosomal location of the mouse long-chain acyl-CoA dehydrogenase gene.

Hinsdale ME, Farmer SC, Johnson KR, Davisson MT, Hamm DA, Tolwani RJ, Wood PA.

Department of Comparative Medicine, School of Medicine, University of Alabama at Birmingham 35294-0019, USA.

The cDNA for mouse long-chain acyl-CoA dehydrogenase (Acadl, gene symbol; LCAD, enzyme) was cloned and characterized. The cDNA was obtained by library screening and reverse transcription-polymerase chain reaction (RT-PCR). The deduced amino acid sequence showed a high degree of homology to both the rat and the human LCAD sequence. Northern analysis of multiple tissues using the mouse Acadl cDNA as a probe showed two bands in all tissues examined. We found a total of three distinct mRNAs for Acadl. These three mRNAs were encoded by a single gene that we mapped to mouse chromosome 1. The three transcripts differed in the 3' untranslated region due to use of alternative polyadenylation sites. Quantitative evaluation of a multitissue Northern blot showed a varied ratio of the larger transcript as compared with the smaller transcripts.

PMID: 8530022 [PubMed - indexed for MEDLINE]