Ribozyme-mediated modulation of human O6-methylgua...[Cancer Res. 1993]

SearchDatabase nameforSearch term

Advanced Search

Your browser version may not work well with NCBI's Web applications. More information here...

Display Show

All: 1

Review: 0

Click to change filter selection through MyNCBI.

1: Cancer Res. 1993 Apr 15;53(8):1731-4. Links

Ribozyme-mediated modulation of human O6-methylguanine-DNA methyltransferase expression.

Potter PM, Harris LC, Remack JS, Edwards CC, Brent TP.

Department of Molecular Pharmacology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.

A synthetic oligonucleotide containing ribozyme sequences targeted to the 5' region of the human O6-methylguanine-DNA methyltransferase (MGMT) mRNA has been constructed. This ribozyme demonstrates cleavage activity in vitro in the presence of Mg2+. To determine whether this ribozyme can function in vivo, HeLa CCL2 cells were transfected with a mammalian expression vector containing the ribozyme sequence. Following selection and expansion of individual transfectants, a stable clone was isolated that lacks both MGMT mRNA and protein. Molecular analysis of this cell line indicates that in vivo cleavage of MGMT mRNA is responsible for the lack of MGMT activity.

PMID: 8467487 [PubMed - indexed for MEDLINE]

Modulation of 1,3-bis-(2-chloroethyl)-1-nitrosourea resistance in human tumor cells using hammerhead ribozymes designed to degrade O6-methylguanine DNA methyltransferase mRNA.
J Pharmacol Exp Ther. 2001 Jul; 298(1):141-7.

[J Pharmacol Exp Ther. 2001]
Graded methylation in the promoter and body of the O6-methylguanine DNA methyltransferase (MGMT) gene correlates with MGMT expression in human glioma cells.
J Biol Chem. 1994 Jun 24; 269(25):17228-37.

[J Biol Chem. 1994]
Targeting of O6-methylguanine-DNA methyltransferase (MGMT) activity by antimessenger oligonucleotide sensitizes CHO/Mex+ transfected cells to mitozolomide.
Carcinogenesis. 1996 Jan; 17(1):25-9.

[Carcinogenesis. 1996]
ReviewCorrelation of O6-methylguanine methyltransferase (MGMT) promoter methylation with clinical outcomes in glioblastoma and clinical strategies to modulate MGMT activity.
J Clin Oncol. 2008 Sep 1; 26(25):4189-99.

[J Clin Oncol. 2008]
ReviewStructure, function, and inhibition of O6-alkylguanine-DNA alkyltransferase.
Prog Nucleic Acid Res Mol Biol. 1995; 51:167-223.

[Prog Nucleic Acid Res Mol Biol. 1995]
» See reviews... | » See all...

Cited by 2 PubMed Central articles

ReviewDNA binding, nucleotide flipping, and the helix-turn-helix motif in base repair by O6-alkylguanine-DNA alkyltransferase and its implications for cancer chemotherapy.
Tubbs JL, Pegg AE, Tainer JA. DNA Repair (Amst). 2007 Aug 1; 6(8):1100-15. Epub 2007 May 7.

[DNA Repair (Amst). 2007]
Ribozyme mediated degradation of beta-amyloid peptide precursor mRNA in COS-7 cells.
Denman RB, Smedman M, Ju W, Rubenstein R, Potempska A, Miller DL. Nucleic Acids Res. 1994 Jun 25; 22(12):2375-82.

[Nucleic Acids Res. 1994]

Recent Activity

Clear Turn Off Turn On

Ribozyme-mediated modulation of human O6-methylguanine-DNA methyltransferase expression.Ribozyme-mediated modulation of human O6-methylguanine-DNA methyltransferase expression.

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

Display Show

Ribozyme-mediated modulation of human O6-methylguanine-DNA methyltransferase expression.

Related articles

Cited by 2 PubMed Central articles

Recent Activity