Rainbow trout ovarian cholesterol side-chain cleav...[FEBS Lett. 1993]

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1: FEBS Lett. 1993 Mar 15;319(1-2):45-8. Links

Rainbow trout ovarian cholesterol side-chain cleavage cytochrome P450 (P450scc). cDNA cloning and mRNA expression during oogenesis.

Takahashi M, Tanaka M, Sakai N, Adachi S, Miller WL, Nagahama Y.

Department of Molecular Biomechanics, Graduate University for Advanced Studies, Okazaki, Japan.

A cDNA clone encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contains an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acids. The predicted amino acid sequence of trout P450scc shows 48% homology with that of human, and 46% homology with that of rat, bovine and pig. P450scc activity was confirmed by transfected COS-1 monkey kidney tumour cells with an expression vector for trout P450scc cDNA and subsequent detection of conversion from 25-hydroxycholesterol to pregnenolone by radioimmunoassay. The cDNA only hybridized to a single 1.8 kb RNA transcript. The transcript was not found in early vitellogenic follicles, barely detected in postvitellogenic follicles, and abundant in postovulatory follicles.

PMID: 8454060 [PubMed - indexed for MEDLINE]

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