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Cloning, DNA sequencing and expression of (3-17)beta hydroxysteroid dehydrogenase from Pseudomonas testosteroni.
Laboratoire de Biochimie, Faculté de Médecine, Brest, France.
We describe the cloning, sequencing and overexpression of the (3-17)beta hydroxysteroid dehydrogenase gene of Pseudomonas testosteroni. A genomic library of Ps. testosteroni total DNA constructed from SauIIIA digests ligated to a lambda gt11 vector was probed with polyclonal antibody raised against purified enzyme. Subclones derived from a recombinant phage containing a 2661-base-pair insert were sequenced and found to contain an open reading frame of 765 base pairs that corresponds to a protein of 254 amino acid residues. A 1492-base-pair fragment was inserted into pBR322 plasmid vector and used to construct a strain of E. coli HB101 that overexpressed the steroid dehydrogenase gene.
PMID: 8382516 [PubMed - indexed for MEDLINE]
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Cited by 5 PubMed Central articles
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TeiR, a LuxR-type transcription factor required for testosterone degradation in Comamonas testosteroni.
Pruneda-Paz JL, Linares M, Cabrera JE, Genti-Raimondi S.
J Bacteriol. 2004 Mar; 186(5):1430-7.
[J Bacteriol. 2004]
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A new bacterial steroid degradation gene cluster in Comamonas testosteroni TA441 which consists of aromatic-compound degradation genes for seco-steroids and 3-ketosteroid dehydrogenase genes.
Horinouchi M, Hayashi T, Yamamoto T, Kudo T.
Appl Environ Microbiol. 2003 Aug; 69(8):4421-30.
[Appl Environ Microbiol. 2003]
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Gene encoding the hydrolase for the product of the meta-cleavage reaction in testosterone degradation by Comamonas testosteroni.
Horinouchi M, Hayashi T, Koshino H, Yamamoto T, Kudo T.
Appl Environ Microbiol. 2003 Apr; 69(4):2139-52.
[Appl Environ Microbiol. 2003]
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