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1: Antimicrob Agents Chemother. 1993 Jul;37(7):1400-5.Click here to read Click here to read Links

Characterization of the gene for chromosomal trimethoprim-sensitive dihydrofolate reductase of Staphylococcus aureus ATCC 25923.

Dale GE, Then RL, Stüber D.

Department of New Technologies, F. Hoffmann-La Roche Ltd., Basel, Switzerland.

The gene for the trimethoprim-sensitive (Tmps) chromosomal dihydrofolate reductase (DHFR) of Staphylococcus aureus ATCC 25923 was cloned and characterized. The structural gene encodes a polypeptide of 159 amino acid residues and has a calculated molecular weight of 18,251. The amino acid sequences of this Tmps DHFR and those of the trimethoprim-resistant type S1 DHFR encoded by transposon Tn4003 are 80% identical. In contrast to the trimethoprim-resistant enzyme, the Tmps DHFR can be highly overexpressed in Escherichia coli, with most of the recombinant protein occurring in a soluble and an active form.

PMID: 8363365 [PubMed - indexed for MEDLINE]

PMCID: PMC187982

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  • Trimethoprim (Proloprim® )

    Trimethoprim eliminates bacteria that cause urinary tract infections. It is used in combination with other drugs to treat certain types of pneumonia. It also is used to treat 'travelers' diarrhea.' Antibiotics will not w...

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