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Sequence of the gene coding for the neurotoxin of Clostridium botulinum type A associated with infant botulism: comparison with other clostridial neurotoxins.
Department of Microbiology, AFRC Institute of Food Research, Reading, UK.
The neurotoxin gene from a strain of Clostridium botulinum type A causing infant botulism was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated using primers designed to conserved regions of published botulinal toxin (BoNT) sequences. Translation of the nucleotide sequence derived from cloned PCR fragments demonstrated that the toxin gene encodes a protein of 1,296 amino acid residues. Comparative alignment of the derived infant BoNT/A sequence with those of other published neurotoxins revealed highest sequence relatedness with BoNT/A of classical food-borne botulism. The sequence identity between infant and classical BoNT/A was 94.9% for the light chain (corresponding to 23 amino acid changes) and 87.1% for the heavy chain (corresponding to 109 amino acid changes).
PMID: 8310180 [PubMed - indexed for MEDLINE]
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Cited by 4 PubMed Central articles
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Genetic diversity among Botulinum Neurotoxin-producing clostridial strains.
Hill KK, Smith TJ, Helma CH, Ticknor LO, Foley BT, Svensson RT, Brown JL, Johnson EA, Smith LA, Okinaka RT, et al.
J Bacteriol. 2007 Feb; 189(3):818-32. Epub 2006 Nov 17.
[J Bacteriol. 2007]
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Sequence variation within botulinum neurotoxin serotypes impacts antibody binding and neutralization.
Smith TJ, Lou J, Geren IN, Forsyth CM, Tsai R, Laporte SL, Tepp WH, Bradshaw M, Johnson EA, Smith LA, et al.
Infect Immun. 2005 Sep; 73(9):5450-7.
[Infect Immun. 2005]
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Differentiation of the gene clusters encoding botulinum neurotoxin type A complexes in Clostridium botulinum type A, Ab, and A(B) strains.
Franciosa G, Floridi F, Maugliani A, Aureli P.
Appl Environ Microbiol. 2004 Dec; 70(12):7192-9.
[Appl Environ Microbiol. 2004]
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