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1: Biochim Biophys Acta. 1994 Jan 4;1183(3):557-61.Links

Cloning and sequencing of the gene encoding spinach ferredoxin-dependent glutamate synthase.

Nalbantoglu B, Hirasawa M, Moomaw C, Nguyen H, Knaff DB, Allen R.

Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409-1061.

The nucleotide sequences of two cDNA clones, totalling 4948 bp in length, encoding 98% of the 1483 amino acids of the mature form of the ferredoxin-dependent glutamate synthase of spinach chloroplasts have been determined. The amino-terminal sequence of the enzyme has been determined by direct sequencing of the protein. The deduced amino-acid sequence of the spinach enzyme is 83% identical to that of the ferredoxin-dependent maize enzyme and shows significant sequence homology to two prokaryotic NAD(P)H-dependent glutamate synthases. Analysis of spinach genomic DNA indicates the presence of a single-copy gene for the spinach enzyme. Northern analysis reveals the presence of a single 5.5 kb transcript, which is present in higher levels in young spinach leaves than in older leaves.

PMID: 8286406 [PubMed - indexed for MEDLINE]