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Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tails.
Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141-9100.
Monocyte chemoattractant protein 1 (MCP-1) is a member of the chemokine family of cytokines that mediate leukocyte chemotaxis. The potent and specific activation of monocytes by MCP-1 may mediate the monocytic infiltration of tissues in atherosclerosis and other inflammatory diseases. We have isolated cDNAs that encode two MCP-1-specific receptors with alternatively spliced carboxyl tails. Expression of the receptors in Xenopus oocytes conferred robust mobilization of intracellular calcium in response to nanomolar concentrations of MCP-1 but not to related chemokines. The MCP-1 receptors are most closely related to the receptor for the chemokines macrophage inflammatory protein 1 alpha and RANTES (regulated on activation, normal T expressed and secreted). The identification of the MCP-1 receptor and cloning of two distinct isoforms provide powerful tools for understanding the specificity and signaling mechanisms of this important chemokine.
PMID: 8146186 [PubMed - indexed for MEDLINE]
PMCID: PMC43448
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Cited by 70 PubMed Central articles
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CCR2-64I and CCR5Delta32 Polymorphisms in Korean Patients with Myasthenia Gravis.
Kim HS, Kim DS, Lee EY, Sunwoo IN, Choi YC.
J Clin Neurol. 2007 Sep; 3(3):133-8. Epub 2007 Sep 20.
[J Clin Neurol. 2007]
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Chemokines: structure, receptors and functions. A new target for inflammation and asthma therapy?
Acker FA, Voss HP, Timmerman H.
Mediators Inflamm. 1996; 5(6):393-416.
[Mediators Inflamm. 1996]
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The monocyte chemoattractant protein-1/cognate CC chemokine receptor 2 system affects cell motility in cultured human podocytes.
Burt D, Salvidio G, Tarabra E, Barutta F, Pinach S, Dentelli P, Camussi G, Perin PC, Gruden G.
Am J Pathol. 2007 Dec; 171(6):1789-99. Epub 2007 Nov 30.
[Am J Pathol. 2007]
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