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Cloning and sequence determination of the gene encoding sorbitol dehydrogenase from Saccharomyces cerevisiae.
Molecular Biology, Abbott Laboratories, Abbott Park, IL 60064.
The identification of a sorbitol-induced sorbitol dehydrogenase (SDH) activity from Saccharomyces cerevisiae is described. The SDH1 structural gene was isolated from a lambda gt11 yeast genomic library using an antibody to a 40-kDa protein induced in yeast cells growing in medium containing sorbitol. The gene encodes a 357-amino-acid (aa) protein deduced from the nucleotide sequence. Comparison of the aa sequence of the yeast SDH1 with that of sheep liver SDH reveals a 63% overall similarity. Yeast transformants containing the cloned gene carried on a multicopy plasmid express high levels of SDH1 only when grown on sorbitol, suggesting that the cloned gene contains both regulatory and coding sequences.
PMID: 8125328 [PubMed - indexed for MEDLINE]
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Cited by 5 PubMed Central articles
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Metabolic engineering of Saccharomyces cerevisiae for conversion of D-glucose to xylitol and other five-carbon sugars and sugar alcohols.
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[Appl Environ Microbiol. 2007]
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Toivari MH, Salusjärvi L, Ruohonen L, Penttilä M.
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[Appl Environ Microbiol. 2004]
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[Plant Physiol. 2004]
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