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Cloning and nucleotide sequence of the Butyrivibrio fibrisolvens gene encoding a type III glutamine synthetase.
Department of Microbiology, University of Cape Town, Rondebosch, South Africa.
A Butyrivibrio fibrisolvens glnA gene encoding glutamine synthetase (GS) was cloned on a recombinant plasmid pGS4 which enabled Escherichia coli glnA deletion mutants to utilize (NH4)2SO4 as a sole source of nitrogen. The nucleotide sequence of a 2423 bp DNA segment containing the GS-coding region of B. fibrisolvens was determined and the complete amino acid sequence (701 residues) was deduced. Comparisons of the derived B. fibrisolvens GS protein sequence with the amino acid sequences of GS from other bacteria indicate that it is the second reported example of a type III GS, originally identified in the obligate anaerobe Bacteroides fragilis. The presence of GS in B. fibrisolvens cells and the regulation of the cloned GS in E. coli cells was demonstrated by Western blot analysis.
PMID: 8103789 [PubMed - indexed for MEDLINE]
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Cited by 6 PubMed Central articles
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Lengsin is a survivor of an ancient family of class I glutamine synthetases re-engineered by evolution for a role in the vertebrate lens.
Wyatt K, White HE, Wang L, Bateman OA, Slingsby C, Orlova EV, Wistow G.
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[Structure. 2006]
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Distribution and evolution of the xylanase genes xynA and xynB and their homologues in strains of Butyrivibrio fibrisolvens.
Dalrymple BP, Swadling Y, Layton I, Gobius KS, Xue GP.
Appl Environ Microbiol. 1999 Aug; 65(8):3660-7.
[Appl Environ Microbiol. 1999]
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ReviewArchaea and the prokaryote-to-eukaryote transition.
Brown JR, Doolittle WF.
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[Microbiol Mol Biol Rev. 1997]
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