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Molecular cloning and characterization of the mouse medium-chain acyl-CoA dehydrogenase cDNA.
Department of Comparative Medicine, School of Medicine, University of Alabama at Birmingham 35294-0019.
Medium-chain acyl-CoA dehydrogenase (MCAD) is one of the three straight-chain length-specific dehydrogenases involved in the first step of fatty acid oxidation. Inherited defects of acyl-CoA dehydrogenases occur in humans, and MCAD deficiency is the most common. We have cloned the coding and 3' untranslated sequence of mouse MCAD cDNA. The mouse MCAD cDNA coding region is 1263 bp long with a 3' untranslated region of 576 bp and encodes a 421 amino acid precursor protein. Comparing the nucleotide and deduced amino acid sequences of the mouse MCAD cDNA to rat and human MCAD cDNAs reveals considerable similarity between species. Amino acid residues where substitutions result in human MCAD deficiency are conserved in the mouse. Amino acid residues involved in important enzymatic functions are also conserved.
PMID: 7829081 [PubMed - indexed for MEDLINE]
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Cited by 3 PubMed Central articles
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Medium-chain acyl-CoA dehydrogenase deficiency in gene-targeted mice.
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PLoS Genet. 2005 Aug; 1(2):e23. Epub 2005 Aug 19.
[PLoS Genet. 2005]
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A gender-related defect in lipid metabolism and glucose homeostasis in peroxisome proliferator- activated receptor alpha- deficient mice.
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J Clin Invest. 1998 Sep 15; 102(6):1083-91.
[J Clin Invest. 1998]
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Transcriptional control of a nuclear gene encoding a mitochondrial fatty acid oxidation enzyme in transgenic mice: role for nuclear receptors in cardiac and brown adipose expression.
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[Mol Cell Biol. 1996]