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1: Biochem Biophys Res Commun. 1994 Dec 30;205(3):1785-9.Click here to read Links
Erratum in:
Biochem Biophys Res Commun. 1995 Feb 27;207(3):1059.

Human proteasome subunits from 2-dimensional gels identified by partial sequencing.

August Krogh Institute, University of Copenhagen, Denmark.

Human proteasomes consist of 14 major subunits which can be resolved by two-dimensional polyacrylamide gel electrophoresis. Tryptic peptides from the subunits were sequenced. This allowed identification of all proteins in two-dimensional electrophoresis gels with proteasome subunits whose sequences are known from cDNA. The results also precisely define the specificity of a panel of subunit-specific monoclonal antibodies. A new proteasome subunit (Z) was discovered. In contrast, the putative subunit MECL-1 could not be detected in human placenta proteasomes.

PMID: 7811265 [PubMed - indexed for MEDLINE]