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- Erratum in:
- Science 1995 Oct 20;270(5235):365.
Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase.
Fred Hutchinson Cancer Research Center, Program in Structural Biology, Seattle, WA 98104, USA.
Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution were used to solve the structures of two sequential intermediates formed during the catalytic actions of isocitrate dehydrogenase. Both intermediates are distinct from the enzyme-substrate and enzyme-product complexes. Mutation of key catalytic residues changed the rate determining steps so that protein and substrate intermediates within the overall reaction pathway could be visualized.
PMID: 7761851 [PubMed - indexed for MEDLINE]
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Structures reported by this article