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1: Biochem Biophys Res Commun. 1995 Jul 17;212(2):705-11.Click here to read Links

Homology cloning of GTP-cyclohydrolase I from various unrelated eukaryotes by reverse-transcription polymerase chain reaction using a general set of degenerate primers.

Maier J, Witter K, Gütlich M, Ziegler I, Werner T, Ninnemann H.

Institut für Chemische Pflanzenphysiologie/Pflanzenbiochemie, Universität Tübingen, Germany.

GTP-cyclohydrolase I is the primary enzyme of tetrahydrobiopterin and folic acid biosynthesis. cDNA fragments of GTP-cyclohydrolase I were obtained from rainbow trout, chicken, the fungi Neurospora crassa, Phycomyces blakesleeanus and Saccharomyces cerevisiae, the cellular slime mold Dictyostelium discoideum, the phytoflagellate Euglena gracilis and the higher plant Mucuna hassjo using primers specific for conserved regions of the open reading frame and the reverse transcription polymerase chain reaction (RT-PCR) technique. A number of regions were found to be strictly conserved between unrelated eukaryotes. These regions may be essential for the function of GTP-cyclohydrolase I and are discussed with respect to the recently resolved crystal structure of the Escherichia coli enzyme.

PMID: 7542887 [PubMed - indexed for MEDLINE]

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