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DNA sequence of a plasmid-encoded dihydrofolate reductase.
The sequence of the methotrexate-resistant dihydrofolate reductase (DHFR) gene borne by the plasmid R-388 was determined. The gene was subcloned and mapped by an in vitro mutagenesis method involving insertion of synthetic oligonucleotide decamers encoding the BamHI recognition site. Sites of insertion that destroyed the methotrexate resistance fell in two regions separated by 300 bp within a 1.2 kb fragment. One of these regions encodes a 78 amino acid polypeptide homologous to another drug-resistant DHFR. The second region essential for DHFR expression appears to be the promoter of the DHFR gene.
PMID: 7022127 [PubMed - indexed for MEDLINE]
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Cited by 32 PubMed Central articles
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Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids.
Revilla C, Garcillán-Barcia MP, Fernández-López R, Thomson NR, Sanders M, Cheung M, Thomas CM, de la Cruz F.
Antimicrob Agents Chemother. 2008 Apr; 52(4):1472-80. Epub 2008 Feb 11.
[Antimicrob Agents Chemother. 2008]
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The Sphingomonas plasmid pCAR3 is involved in complete mineralization of carbazole.
Shintani M, Urata M, Inoue K, Eto K, Habe H, Omori T, Yamane H, Nojiri H.
J Bacteriol. 2007 Mar; 189(5):2007-20. Epub 2006 Dec 15.
[J Bacteriol. 2007]
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New integron-associated gene cassette encoding a trimethoprim-resistant DfrB-type dihydrofolate reductase.
Levings RS, Lightfoot D, Elbourne LD, Djordjevic SP, Hall RM.
Antimicrob Agents Chemother. 2006 Aug; 50(8):2863-5.
[Antimicrob Agents Chemother. 2006]
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Patient Drug Information
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Methotrexate (Rheumatrex® , Trexall® )
Methotrexate is used to treat severe psoriasis (a skin disease in which red, scaly patches form on some areas of the body) that cannot be controlled by other treatments. Methotrexate is also used along with rest, physica...