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Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX.
A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisely one codon has been deleted.Images
PMID: 6687940 [PubMed - indexed for MEDLINE]
PMCID: PMC325887
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Cited by 36 PubMed Central articles
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Monoclonal antibodies to coagulation factor IX define a high-frequency polymorphism by immunoassays.
Smith KJ.
Am J Hum Genet. 1985 Jul; 37(4):668-79.
[Am J Hum Genet. 1985]
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Cytological mapping of the human glucose-6-phosphate dehydrogenase gene distal to the fragile-X site suggests a high rate of meiotic recombination across this site.
Szabo P, Purrello M, Rocchi M, Archidiacono N, Alhadeff B, Filippi G, Toniolo D, Martini G, Luzzatto L, Siniscalco M.
Proc Natl Acad Sci U S A. 1984 Dec; 81(24):7855-9.
[Proc Natl Acad Sci U S A. 1984]
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Isolation of a genomic clone for bovine pancreatic trypsin inhibitor by using a unique-sequence synthetic DNA probe.
Anderson S, Kingston IB.
Proc Natl Acad Sci U S A. 1983 Nov; 80(22):6838-42.
[Proc Natl Acad Sci U S A. 1983]
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