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Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli.
We describe a method for the formation of hybrid genes by in vivo recombination between two genes with partial sequence homology. DNA structures consisting of plasmid vector sequences, flanked by the alpha 2 interferon gene on the one side and a portion of the alpha 1 interferon gene (homology about 80%) on the other, were transfected into E. coli SK1592. Appropriate resistance markers allowed the isolation of colonies containing circular plasmids which arose by in vivo recombination between the partly homologous interferon gene sequences. Eleven different recombinant genes were identified, six of which encoded new hybrid interferons not easily accessible by recombinant DNA techniques.
PMID: 6310510 [PubMed - indexed for MEDLINE]
PMCID: PMC326304
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Cited by 14 PubMed Central articles
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Chimeric transcriptional activators generated in vivo from VnfA and AnfA of Azotobacter vinelandii: N-terminal domain of AnfA is responsible for dependence on nitrogenase Fe protein.
Frise E, Green A, Drummond M.
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[J Bacteriol. 1994]
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Mechanism of intramolecular recyclization and deletion formation following transformation of Escherichia coli with linearized plasmid DNA.
Conley EC, Saunders VA, Jackson V, Saunders JR.
Nucleic Acids Res. 1986 Nov 25; 14(22):8919-32.
[Nucleic Acids Res. 1986]
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Simultaneous synthesis of human-, mouse- and chimeric epidermal growth factor genes via 'hybrid gene synthesis' approach.
Sung WL, Zahab DM, Yao FL, Wu R, Narang SA.
Nucleic Acids Res. 1986 Aug 11; 14(15):6159-68.
[Nucleic Acids Res. 1986]
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