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Cloned cDNA to cholecystokinin mRNA predicts an identical preprocholecystokinin in pig brain and gut.
Molecular cloning has established the structure of preprocholecystokinin in porcine cerebral cortex and duodenal mucosa. This precursor is 114 amino acids long, is identical in brain and gut, contains all the cholecystokinin (CCK) peptides previously isolated, and has the characteristics of a prohormone. It contains a putative amino-terminal signal peptide, basic processing sites, and a carboxyl-terminal amidation signal. The CCK mRNAs from brain and gut are approximately 850 nucleotides long and differ by only a few single base changes. This analysis establishes by a strict criterion that CCK is synthesized in both brain and gut and that the different distributions of molecular forms of CCK in the two tissues are most probably a consequence of tissue-specific posttranslational events.
PMID: 6205394 [PubMed - indexed for MEDLINE]
PMCID: PMC345577
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Cited by 8 PubMed Central articles
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Complete nucleotide sequence of mRNA for caerulein precursor from Xenopus skin: the mRNA contains an unusual repetitive structure.
Wakabayashi T, Kato H, Tachibana S.
Nucleic Acids Res. 1985 Mar 25; 13(6):1817-28.
[Nucleic Acids Res. 1985]
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Differential expression of the mouse cholecystokinin gene during brain and gut development.
Friedman J, Schneider BS, Powell D.
Proc Natl Acad Sci U S A. 1985 Sep; 82(17):5593-7.
[Proc Natl Acad Sci U S A. 1985]
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Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine.
Takahashi Y, Kato K, Hayashizaki Y, Wakabayashi T, Ohtsuka E, Matsuki S, Ikehara M, Matsubara K.
Proc Natl Acad Sci U S A. 1985 Apr; 82(7):1931-5.
[Proc Natl Acad Sci U S A. 1985]
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