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The sequence of human serum albumin cDNA and its expression in E. coli.
A recombinant plasmid has been constructed which contains the mature protein coding region of the human serum albumin (HSA) gene. Bacteria containing this plasmid synthesize HSA protein under control of the E. coli trp promoter-operator. The DNA sequence and predicted protein sequence of HSA were determined from the cDNA plasmid and are compared to existing data obtained from direct protein sequencing. The DNA sequence predicts a mature protein of 585 amino acids preceded by a 24 amino acid "prepro" peptide.
PMID: 6171778 [PubMed - indexed for MEDLINE]
PMCID: PMC327587
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Cited by 36 PubMed Central articles
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Cloning of several cDNA segments coding for human liver proteins.
Costanzo F, Castagnoli L, Dente L, Arcari P, Smith M, Costanzo P, Raugei G, Izzo P, Pietropaolo TC, Bougueleret L, et al.
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[EMBO J. 1983]
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Photoaffinity labeling probe for the substrate binding site of human phenol sulfotransferase (SULT1A1): 7-azido-4-methylcoumarin.
Chen G, Battaglia E, Senay C, Falany CN, Radominska-Pandya A.
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[Protein Sci. 1999]
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Use of the KlADH4 promoter for ethanol-dependent production of recombinant human serum albumin in Kluyveromyces lactis.
Saliola M, Mazzoni C, Solimando N, Crisà A, Falcone C, Jung G, Fleer R.
Appl Environ Microbiol. 1999 Jan; 65(1):53-60.
[Appl Environ Microbiol. 1999]
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