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Human leukocyte interferon produced by E. coli is biologically active.
Goeddel DV,
Yelverton E,
Ullrich A,
Heyneker HL,
Miozzari G,
Holmes W,
Seeburg PH,
Dull T,
May L,
Stebbing N,
Crea R,
Maeda S,
McCandliss R,
Sloma A,
Tabor JM,
Gross M,
Familletti PC,
Pestka S.
A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli. The DNA sequence codes for a 23-amino acid signal peptide followed by an interferon polypeptide of 165 amino acids. An expression plasmid was constructed which permits the synthesis in E. coli of 2.5 x 10(8) units of interferon per litre of culture. This LeIF protected squirrel monkeys from lethal encephalomyocarditis virus infection.
PMID: 6159538 [PubMed - indexed for MEDLINE]
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Cited by 69 PubMed Central articles
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ReviewThe rationale for the current boom in anti-TNFalpha treatment. Is there an effective means to define therapeutic targets for drugs that provide all the benefits of anti-TNFalpha and minimise hazards?
Feldmann M, Bondeson J, Brennan FM, Foxwell BM, Maini RN.
Ann Rheum Dis. 1999 Nov; 58 Suppl 1:I27-31.
[Ann Rheum Dis. 1999]
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Polymorphism in the interferon-alpha gene family.
Golovleva I, Kandefer-Szerszen M, Beckman L, Lundgren E.
Am J Hum Genet. 1996 Sep; 59(3):570-8.
[Am J Hum Genet. 1996]
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Periplasmic expression of human interferon-alpha 2c in Escherichia coli results in a correctly folded molecule.
Voss T, Falkner E, Ahorn H, Krystek E, Maurer-Fogy I, Bodo G, Hauptmann R.
Biochem J. 1994 Mar 15; 298 Pt 3:719-25.
[Biochem J. 1994]
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