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Sequence heterogeneity of murine complementary DNA clones related to the C4 and C4-Slp isoforms of the fourth complement component.
Two classes of mRNA encoding the murine C4 protein were identified by sequence analysis of clones isolated from a liver complementary DNA library. The divergence found within a 357 base pair sequence available for comparison is limited to five nucleotide replacements located in the region corresponding to the carboxy-terminal end of the C4d peptide fragment. One of the nucleotide substitutions influences the presence of a site for the Hind III restriction endonuclease. That this restriction site indeed discriminates the two non-allelic genes encoding the mouse C4 and C4-Slp isoforms has been demonstrated by Southern blot analysis and nucleotide sequencing at the genomic level. Circumstantial evidence supports the identification of the gene lacking the Hind III site in the region corresponding to the carboxy-terminal end of the C4d fragment as the one encoding the C4-Slp isotype.
PMID: 6149581 [PubMed - indexed for MEDLINE]
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Cited by 3 PubMed Central articles
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Liver mRNA probes disclose two cytochrome P-450 genes duplicated in tandem with the complement C4 loci of the mouse H-2S region.
Amor M, Tosi M, Duponchel C, Steinmetz M, Meo T.
Proc Natl Acad Sci U S A. 1985 Jul; 82(13):4453-7.
[Proc Natl Acad Sci U S A. 1985]
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Multiple duplications of complement C4 gene correlate with H-2-controlled testosterone-independent expression of its sex-limited isoform, C4-Slp.
Levi-Strauss M, Tosi M, Steinmetz M, Klein J, Meo T.
Proc Natl Acad Sci U S A. 1985 Mar; 82(6):1746-50.
[Proc Natl Acad Sci U S A. 1985]
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Orientation and molecular map position of the complement genes in the mouse MHC.
Müller U, Stephan D, Philippsen P, Steinmetz M.
EMBO J. 1987 Feb; 6(2):369-73.
[EMBO J. 1987]