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cDNA and gene nucleotide sequence of porcine plasminogen activator.
We have isolated cDNA and genomic clones coding for porcine plasminogen activator (urokinase, uPA). The cDNA is 2375 nucleotides long: it consists of a 5'-non-coding region (104 nucleotides), an open reading frame of 1329 nucleotides, and 3'-non-coding region of 942 nucleotides apart from the poly A tail. The genomic segment corresponding to the transcribed sequence is 5.85 kb long; it is composed of 11 exons and 10 introns. The 5'-flanking genomic region contains a number of sequences of potential regulatory significance, including possible hormone receptor binding sites and a sequence which we tentatively propose may be involved in activation of transcription by cAMP. The full sequence of both cDNA and genomic clones, the latter including 1.3 kb of flanking region, is presented and discussed, and the deduced amino acid sequence compared with that of human uPA.
PMID: 6096832 [PubMed - indexed for MEDLINE]
PMCID: PMC320478
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Cited by 23 PubMed Central articles
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Cytoskeletal reorganization and TPA differently modify AP-1 to induce the urokinase-type plasminogen activator gene in LLC-PK1 cells.
Lee JS, von der Ahe D, Kiefer B, Nagamine Y.
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[Nucleic Acids Res. 1993]
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Nanbu R, Menoud PA, Nagamine Y.
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[Mol Cell Biol. 1994]
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Kaufman RJ, Wasley LC, Spiliotes AJ, Gossels SD, Latt SA, Larsen GR, Kay RM.
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[Mol Cell Biol. 1985]
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