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Rabbit muscle creatine phosphokinase. CDNA cloning, primary structure and detection of human homologues.
Putney S,
Herlihy W,
Royal N,
Pang H,
Aposhian HV,
Pickering L,
Belagaje R,
Biemann K,
Page D,
Kuby S, et al.
A cDNA library was constructed from rabbit muscle poly(A) RNA. Limited amino acid sequence information was obtained on rabbit muscle creatine phosphokinase and this was the basis for design and synthesis of two oligonucleotide probes complementary to a creatine kinase cDNA sequence which encodes a pentapeptide. Colony hybridizations with the probes and subsequent steps led to isolation of two clones, whose cDNA segments partially overlap and which together encode the entire protein. The primary structure was established from the sequence of two cDNA clones and from independently determined sequences of scattered portions of the polypeptide. The reactive cysteine has been located to position 282 within the 380 amino acid polypeptide. The rabbit cDNA hybridizes to digests of human chromosomal DNA. This reveals a restriction fragment length polymorphism associated with the human homologue(s) which hybridizes to the rabbit cDNA.
PMID: 6094551 [PubMed - indexed for MEDLINE]
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Cited by 16 PubMed Central articles
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Arginine kinase evolved twice: evidence that echinoderm arginine kinase originated from creatine kinase.
Suzuki T, Kamidochi M, Inoue N, Kawamichi H, Yazawa Y, Furukohri T, Ellington WR.
Biochem J. 1999 Jun 15; 340 ( Pt 3):671-5.
[Biochem J. 1999]
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Unfolding and refolding of dimeric creatine kinase equilibrium and kinetic studies.
Fan YX, Zhou JM, Kihara H, Tsou CL.
Protein Sci. 1998 Dec; 7(12):2631-41.
[Protein Sci. 1998]
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Creatine kinase: essential arginine residues at the nucleotide binding site identified by chemical modification and high-resolution tandem mass spectrometry.
Wood TD, Guan Z, Borders CL Jr, Chen LH, Kenyon GL, McLafferty FW.
Proc Natl Acad Sci U S A. 1998 Mar 31; 95(7):3362-5.
[Proc Natl Acad Sci U S A. 1998]
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