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Characterization of the polypeptide composition of human factor VIII:C and the nucleotide sequence and expression of the human kidney cDNA.
Truett MA,
Blacher R,
Burke RL,
Caput D,
Chu C,
Dina D,
Hartog K,
Kuo CH,
Masiarz FR,
Merryweather JP, et al.
Human coagulation factor VIII:C has been purified approximately 5000-fold from commercial preparations with an average activity yield of 35%. Proteins of 92 kD and 77-80 kD enriched during purification are precipitated by a human serum polyclonal antibody which inhibits factor VIII:C activity. Evidence suggests that these polypeptides are linked by a calcium ion bridge. Partial amino acid sequence information from these proteins has been obtained from the intact polypeptides and from products of digestion with thrombin, endoproteinase lysC, or trypsin after citraconylation. An oligonucleotide probe designed from one of the amino acid sequences was used to isolate a partial genomic clone from a human 4X chromosome library in bacteriophage lambda. The genomic segment was used to isolate two cDNA molecules encompassing the entire human kidney factor VIII:C mRNA. Biologically active factor VIII:C has been produced in a mammalian cell line utilizing a complete cDNA construction.
PMID: 3935400 [PubMed - indexed for MEDLINE]
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Cited by 12 PubMed Central articles
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Transformation-specific interaction of the bovine papillomavirus E5 oncoprotein with the platelet-derived growth factor receptor transmembrane domain and the epidermal growth factor receptor cytoplasmic domain.
Cohen BD, Goldstein DJ, Rutledge L, Vass WC, Lowy DR, Schlegel R, Schiller JT.
J Virol. 1993 Sep; 67(9):5303-11.
[J Virol. 1993]
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Reversion of autocrine transformation by a dominant negative platelet-derived growth factor mutant.
Vassbotn FS, Andersson M, Westermark B, Heldin CH, Ostman A.
Mol Cell Biol. 1993 Jul; 13(7):4066-76.
[Mol Cell Biol. 1993]
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Gene therapy for hemophilia A: production of therapeutic levels of human factor VIII in vivo in mice.
Dwarki VJ, Belloni P, Nijjar T, Smith J, Couto L, Rabier M, Clift S, Berns A, Cohen LK.
Proc Natl Acad Sci U S A. 1995 Feb 14; 92(4):1023-7.
[Proc Natl Acad Sci U S A. 1995]
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