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Nucleotide sequence of the dihydrofolate reductase gene of methotrexate-resistant Lactobacillus casei.
The nucleotide sequence of the dihydrofolate reductase (DHFR) gene of a methotrexate-resistant strain of Lactobacillus casei, which is the source of DHFR for nuclear magnetic resonance (NMR) studies, has been determined. The derived amino acid sequence differs from that obtained by protein sequencing by the presence of aspartic acid instead of asparagine at position 8 and proline instead of leucine at position 90. The nucleotide sequences of 320-bp 5' and 335-bp 3' flanking regions of this gene have also been determined.
PMID: 3928445 [PubMed - indexed for MEDLINE]
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Cited by 6 PubMed Central articles
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Structure and dynamics in solution of the complex of Lactobacillus casei dihydrofolate reductase with the new lipophilic antifolate drug trimetrexate.
Polshakov VI, Birdsall B, Frenkiel TA, Gargaro AR, Feeney J.
Protein Sci. 1999 Mar; 8(3):467-81.
[Protein Sci. 1999]
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3H-n.m.r. studies of multiple conformations and dynamic processes in complexes of folate and methotrexate with Lactobacillus casei dihydrofolate reductase.
Curtis N, Moore S, Birdsall B, Bloxsidge J, Gibson CL, Jones JR, Feeney J.
Biochem J. 1994 Oct 15; 303 ( Pt 2):401-5.
[Biochem J. 1994]
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Cloning and molecular analysis of the dihydrofolate reductase gene from Lactococcus lactis.
Leszczyńska K, Bolhuis A, Leenhouts K, Venema G, Cegłowski P.
Appl Environ Microbiol. 1995 Feb; 61(2):561-6.
[Appl Environ Microbiol. 1995]
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Patient Drug Information
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Methotrexate (Rheumatrex® , Trexall® )
Methotrexate is used to treat severe psoriasis (a skin disease in which red, scaly patches form on some areas of the body) that cannot be controlled by other treatments. Methotrexate is also used along with rest, physica...