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Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.
Three clones for the human tumor antigen p53 were isolated from a cDNA library prepared from A431 cells. One of these clones, pR4-2, contains the entire coding region for human p53. This clone directs the synthesis of a polypeptide with the correct molecular weight and immunological epitopes of an authentic p53 molecule in an in vitro transcription-translation reaction. Although the pR4-2 clone contains the coding region for p53, it is not a full-length copy of the human p53 mRNA. Northern analysis showed that the p53 mRNA is approximately 2,500 nucleotides long, whereas the pR4-2 insert is only 1,760 base pairs in length. Analysis of the DNA sequence of this clone suggests that the human p53 polypeptide has 393 amino acids. We compared the predicted amino acid sequence of the pR4-2 clone with similar clones for the mouse p53 and found long regions of amino acid homology between these two molecules.
PMID: 3894933 [PubMed - indexed for MEDLINE]
PMCID: PMC367278
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Cited by 36 PubMed Central articles
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Human immunodeficiency virus type 1 Nef binds to tumor suppressor p53 and protects cells against p53-mediated apoptosis.
Greenway AL, McPhee DA, Allen K, Johnstone R, Holloway G, Mills J, Azad A, Sankovich S, Lambert P.
J Virol. 2002 Mar; 76(6):2692-702.
[J Virol. 2002]
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Tolerance to p53 by A2.1-restricted cytotoxic T lymphocytes.
Theobald M, Biggs J, Hernández J, Lustgarten J, Labadie C, Sherman LA.
J Exp Med. 1997 Mar 3; 185(5):833-41.
[J Exp Med. 1997]
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The p53 status of Chinese hamster V79 cells frequently used for studies on DNA damage and DNA repair.
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[Nucleic Acids Res. 1997]
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