The amino acid sequence of the trimethoprim-resist...[J Biol Chem. 1979]

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1: J Biol Chem. 1979 Nov 10;254(21):10857-61. Links

The amino acid sequence of the trimethoprim-resistant dihydrofolate reductase specified in Escherichia coli by R-plasmid R67.

Stone D, Smith SL.

The amino acid sequence of a trimethoprim-resistant dihydrofolate reductase (EC 1.5.1.3) specified by the R-plasmid R67 is described. The sequence was deduced from automatic and manual sequence analysis of the intact protein, the fragments produced by cyanogen bromide cleavage, and peptides derived from the largest cyanogen bromide fragment by digestion with trypsin, Staphylococcus aureus V8 proteus, chymotrypsin, and Lysobacter enzymogenes alpha-lytic protease. The complete sequence comprises 78 residues in a single polypeptide chain of molecular weight 8444. No evidence of heterogeneity was obtained, indicating that all subunits of the native enzyme are identical. Comparison of the sequence with that of all known dihydrofolate reductases shows no significant sequence homology.

PMID: 387758 [PubMed - indexed for MEDLINE]

The amino-acid sequence of the dihydrofolate reductase of a trimethoprim-resistant strain of Escherichia coli.
Eur J Biochem. 1977 Feb; 72(3):613-24.

[Eur J Biochem. 1977]
Characterization of a R plasmid-associated, trimethoprim-resistant dihydrofolate reductase and determination of the nucleotide sequence of the reductase gene.
Nucleic Acids Res. 1981 Feb 11; 9(3):697-710.

[Nucleic Acids Res. 1981]
Construction of a synthetic gene for an R-plasmid-encoded dihydrofolate reductase and studies on the role of the N-terminus in the protein.
Biochemistry. 1991 Nov 12; 30(45):10895-904.

[Biochemistry. 1991]
Protein expression in Escherichia coli minicells containing recombinant plasmids specifying trimethoprim-resistant dihydrofolate reductases.
J Bacteriol. 1980 Feb; 141(2):779-85.

[J Bacteriol. 1980]
Identification of the type I trimethoprim-resistant dihydrofolate reductase specified by the Escherichia coli R-plasmid R483: comparison with procaryotic and eucaryotic dihydrofolate reductases.
J Bacteriol. 1983 Sep; 155(3):1001-8.

[J Bacteriol. 1983]
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Cited by 13 PubMed Central articles

A novel dihydrofolate reductase cassette inserted in an integron borne on a Tn21-like element.
Heikkilä E, Skurnik M, Sundström L, Huovinen P. Antimicrob Agents Chemother. 1993 Jun; 37(6):1297-304.

[Antimicrob Agents Chemother. 1993]
ReviewTrimethoprim and sulfonamide resistance.
Huovinen P, Sundström L, Swedberg G, Sköld O. Antimicrob Agents Chemother. 1995 Feb; 39(2):279-89.

[Antimicrob Agents Chemother. 1995]
Protein expression in Escherichia coli minicells containing recombinant plasmids specifying trimethoprim-resistant dihydrofolate reductases.
Fling ME, Elwell LP. J Bacteriol. 1980 Feb; 141(2):779-85.

[J Bacteriol. 1980]
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Structures reported by this article

Structure Of Dihydrofolate Reductase

PDB: 1VIF

Source: Escherichia coli

Method: X-Ray Diffraction | Resolution: 1.8 Å
» See all 2 structures...

Patient Drug Information

Trimethoprim (Proloprim® )
Trimethoprim eliminates bacteria that cause urinary tract infections. It is used in combination with other drugs to treat certain types of pneumonia. It also is used to treat 'travelers' diarrhea.' Antibiotics will not w...
Source: AHFS Consumer Medication Information

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