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Accumulation of creatine kinase mRNA during myogenesis: molecular cloning of a B-creatine kinase cDNA.
Cytosolic creatine kinase isoenzymes MM, MB, and BB are assembled from M or B subunits which occur in different relative amounts in specific tissues. The accumulation of mRNAs encoding the M and B subunits was measured during myogenesis in culture. The relative concentration of the two mRNAs was determined by hybridization with a M-CK cDNA probe isolated previously and a B-CK cDNA probe, the cloning and characterization of which is reported here. The B-CK cDNA hybridizes specifically to a 1.6-kb mRNA found in brain and gizzard but not in adult skeletal muscle tissue. The M-CK cDNA hybridizes to a smaller mRNA 1.4-kb long which is specific to skeletal muscle. In culture, the B-CK mRNA is transiently induced and then declines to a low but detectable level.
PMID: 3840441 [PubMed - indexed for MEDLINE]
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Cited by 3 PubMed Central articles
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Mouse p53 represses the rat brain creatine kinase gene but activates the rat muscle creatine kinase gene.
Zhao J, Schmieg FI, Simmons DT, Molloy GR.
Mol Cell Biol. 1994 Dec; 14(12):8483-92.
[Mol Cell Biol. 1994]
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cDNA cloning and mapping of the human creatine kinase M gene to 19q13.
Nigro JM, Schweinfest CW, Rajkovic A, Pavlovic J, Jamal S, Dottin RP, Hart JT, Kamarck ME, Rae PM, Carty MD.
Am J Hum Genet. 1987 Feb; 40(2):115-25.
[Am J Hum Genet. 1987]
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Multiple positive and negative elements regulate human brain creatine kinase gene expression.
Ritchie ME, Trask RV, Fontanet HL, Billadello JJ.
Nucleic Acids Res. 1991 Nov 25; 19(22):6231-40.
[Nucleic Acids Res. 1991]