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1: Nucleic Acids Res. 1986 Nov 11;14(21):8595-603. Links

Sequencing and identification of a cDNA clone for tomato polygalacturonase.

Grierson D, Tucker GA, Keen J, Ray J, Bird CR, Schuch W.

The 2a isoenzyme of tomato polygalacturonase was purified from ripe fruit and characterised. The N-terminal amino acid sequence of the protein was determined in order to identify polygalacturonase cDNA clones. The nucleotide sequence of a ripening-related cDNA (pTOM 6) was determined and found to encode the N-terminal sequence of mature polygalacturonase 2a. The complete open reading frame encodes a polypeptide of molecular weight 50,051, including a putative pre-sequence of 71 amino acids.

PMID: 3786135 [PubMed - indexed for MEDLINE]

PMCID: PMC311879

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[Biochim Biophys Acta. 1990]
Isolation and characterization of a fruit-specific cDNA and the corresponding genomic clone from tomato.
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[Plant Mol Biol. 1989]
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Cited by 20 PubMed Central articles

Genome mapping and molecular breeding of tomato.
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[Int J Plant Genomics. 2007]
Polygalacturonase Isozymes and Pectin Depolymerization in Transgenic rin Tomato Fruit.
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Reduction of polygalacturonase activity in tomato fruit by antisense RNA.
Sheehy RE, Kramer M, Hiatt WR. Proc Natl Acad Sci U S A. 1988 Dec; 85(23):8805-8809.

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