-
Expression of three mRNA species from a single rat aldolase A gene, differing in their 5' non-coding regions.
The complete nucleotide sequence of the rat aldolase A isozyme gene, including the 5' and 3' flanking sequences, was determined. The gene comprises ten exons, spans 4827 base-pairs and occurs in a single copy per haploid rat genome. The genomic DNA sequence was compared with those of three species of rat aldolase A mRNA (mRNAs I, II and III) that have been found to differ from each other only in the 5' non-coding region and to be expressed tissue-specifically. It revealed that the first exon (exon M1) encodes the 5' non-coding sequence of mRNA I, while the second exon (exon AH1) encodes those of mRNAs II and III and the following eight exons (exons 2 to 9) are shared commonly by all the mRNA species. These results allowed us to conclude that mRNA I and mRNAs II, III were generated from a single aldolase A gene by alternative usage of exon M1 or exon AH1 in addition to exons 2 to 9. S1 nuclease mapping of the 5' ends of their precursor RNAs suggested that these three mRNA species were transcribed from three different initiation sites on the single gene.
PMID: 3783705 [PubMed - indexed for MEDLINE]
-
Cited by 15 PubMed Central articles
-
Histidine to aspartate phosphotransferase activity of nm23 proteins: phosphorylation of aldolase C on Asp-319.
Wagner PD, Vu ND.
Biochem J. 2000 Mar 15; 346 Pt 3:623-30.
[Biochem J. 2000]
-
Genomic sequences of aldolase C (Zebrin II) direct lacZ expression exclusively in non-neuronal cells of transgenic mice.
Walther EU, Dichgans M, Maricich SM, Romito RR, Yang F, Dziennis S, Zackson S, Hawkes R, Herrup K.
Proc Natl Acad Sci U S A. 1998 Mar 3; 95(5):2615-20.
[Proc Natl Acad Sci U S A. 1998]
-
The MEF-3 motif is required for MEF-2-mediated skeletal muscle-specific induction of the rat aldolase A gene.
Hidaka K, Yamamoto I, Arai Y, Mukai T.
Mol Cell Biol. 1993 Oct; 13(10):6469-78.
[Mol Cell Biol. 1993]
- » See all...