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Isolation and characterization of a cDNA clone for porcine thyroid peroxidase.
We undertook the molecular cloning of porcine thyroid peroxidase (TPO). Four oligonucleotide probes were synthesized on the basis of amino acid sequences of 3 tryptic peptides from highly purified porcine TPO. These probes were used to screen a pig thyroid cDNA library. Seven of 16 selected clones (0.45-1.15 kb in size) reacted with all 4 probes. Nucleotide sequencing of the 1.15 kb at the 3'-end of the structural gene revealed the complementary sequence to all 4 probes as well as the nucleotides coding for the entire length of the 3 tryptic peptides. There is an open reading frame of 332 amino acid residues. On Northern blot analysis this gene codes for an mRNA species of 2.85 kb, corresponding to the anticipated size of the mRNA for the intact TPO molecule. We have therefore cloned and characterized a cDNA clone coding for approx. 36% of porcine thyroid peroxidase.
PMID: 3780975 [PubMed - indexed for MEDLINE]
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Cited by 5 PubMed Central articles
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Isolation of a complementary DNA clone for thyroid microsomal antigen. Homology with the gene for thyroid peroxidase.
Seto P, Hirayu H, Magnusson RP, Gestautas J, Portmann L, DeGroot LJ, Rapoport B.
J Clin Invest. 1987 Oct; 80(4):1205-8.
[J Clin Invest. 1987]
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Human thyroid peroxidase: complete cDNA and protein sequence, chromosome mapping, and identification of two alternately spliced mRNAs.
Kimura S, Kotani T, McBride OW, Umeki K, Hirai K, Nakayama T, Ohtaki S.
Proc Natl Acad Sci U S A. 1987 Aug; 84(16):5555-9.
[Proc Natl Acad Sci U S A. 1987]
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Complete nucleotide sequence of the human thyroperoxidase-microsomal antigen cDNA.
Libert F, Ruel J, Ludgate M, Swillens S, Alexander N, Vassart G, Dinsart C.
Nucleic Acids Res. 1987 Aug 25; 15(16):6735.
[Nucleic Acids Res. 1987]
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