Phenotypic expression in E. coli of a DNA sequence...[Nature. 1978]

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1: Nature. 1978 Oct 19;275(5681):617-24.Links

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase.

Chang AC, Nunberg JH, Kaufman RJ, Erlich HA, Schimke RT, Cohen SN.

The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the antimetabolic drug trimethoprim.

PMID: 360074 [PubMed - indexed for MEDLINE]

Nucleotide sequence of dihydrofolate reductase genes from trimethoprim-resistant mutants of Escherichia coli. Evidence that dihydrofolate reductase interacts with another essential gene product.
Mol Gen Genet. 1982; 187(1):72-8.

[Mol Gen Genet. 1982]
Protein expression in Escherichia coli minicells containing recombinant plasmids specifying trimethoprim-resistant dihydrofolate reductases.
J Bacteriol. 1980 Feb; 141(2):779-85.

[J Bacteriol. 1980]
Site-directed mutagenesis of mouse dihydrofolate reductase. Mutants with increased resistance to methotrexate and trimethoprim.
J Biol Chem. 1988 Sep 5; 263(25):12500-8.

[J Biol Chem. 1988]
Identification of the type I trimethoprim-resistant dihydrofolate reductase specified by the Escherichia coli R-plasmid R483: comparison with procaryotic and eucaryotic dihydrofolate reductases.
J Bacteriol. 1983 Sep; 155(3):1001-8.

[J Bacteriol. 1983]
The nucleotide sequence of the trimethoprim-resistant dihydrofolate reductase gene harbored by Tn7.
Nucleic Acids Res. 1983 Aug 11; 11(15):5147-58.

[Nucleic Acids Res. 1983]
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Isolation of cloned cDNAs to auxin-responsive poly(A)RNAs of elongating soybean hypocotyl.
Walker JC, Key JL. Proc Natl Acad Sci U S A. 1982 Dec; 79(23):7185-7189.

[Proc Natl Acad Sci U S A. 1982]
Characterization of human class-switched polymeric (immunoglobulin M [IgM] and IgA) anti-human immunodeficiency virus type 1 antibodies 2F5 and 2G12.
Wolbank S, Kunert R, Stiegler G, Katinger H. J Virol. 2003 Apr; 77(7):4095-103.

[J Virol. 2003]
ReviewStructural biology.
Holmes KC. Philos Trans R Soc Lond B Biol Sci. 1999 Dec 29; 354(1392):1977-84.

[Philos Trans R Soc Lond B Biol Sci. 1999]
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Trimethoprim (Proloprim® )
Trimethoprim eliminates bacteria that cause urinary tract infections. It is used in combination with other drugs to treat certain types of pneumonia. It also is used to treat 'travelers' diarrhea.' Antibiotics will not w...
Source: AHFS Consumer Medication Information

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