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The primary structure of a peroxisomal fatty acyl-CoA oxidase from the yeast Candida tropicalis pK233.
We report the isolation and nucleotide (nt) sequence determination of a gene encoding peroxisomal fatty acyl-CoA oxidase (AOx) from the yeast Candida tropicalis pK233. The AOx gene contains no intervening sequences and has a single open reading frame of 2127 nt encoding a protein of 708 amino acids (aa), not including the initiator methionine. The Mr of the protein is 79,155. Codon utilization in the gene is not random, with 87.4% of the aa specified by 25 principal codons. The principal codons used in the expression of AOx in C. tropicalis are similar to those used in highly expressed genes of Saccharomyces cerevisiae. The AOx protein shows a 94.2% homology with POX4 protein of C. tropicalis. One stretch of 36 aa shows no homology between the two proteins.
PMID: 3596241 [PubMed - indexed for MEDLINE]
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Cited by 4 PubMed Central articles
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Evaluation of acyl coenzyme A oxidase (Aox) isozyme function in the n-alkane-assimilating yeast Yarrowia lipolytica.
Wang HJ, Le Dall MT, Wach Y, Laroche C, Belin JM, Gaillardin C, Nicaud JM.
J Bacteriol. 1999 Sep; 181(17):5140-8.
[J Bacteriol. 1999]
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Peroxisome targeting signal of rat liver acyl-coenzyme A oxidase resides at the carboxy terminus.
Miyazawa S, Osumi T, Hashimoto T, Ohno K, Miura S, Fujiki Y.
Mol Cell Biol. 1989 Jan; 9(1):83-91.
[Mol Cell Biol. 1989]
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Isolation of Candida tropicalis auxotrophic mutants.
Gleeson MA, Haas LO, Cregg JM.
Appl Environ Microbiol. 1990 Aug; 56(8):2562-4.
[Appl Environ Microbiol. 1990]
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