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A ras-like protein is required for a post-Golgi event in yeast secretion.
Secretion is blocked at the post-Golgi stage within 5 min of a shift of sec4-8 cells from 25 degrees C to 37 degrees C. Analysis of SEC4 predicts a protein product of 23.5 kd molecular weight that shares 32% homology with ras proteins and is essential for growth. The regions of best homology are those involved in the binding and hydrolysis of GTP. Duplication of SEC4 suppresses post-Golgi-blocked mutations in three sec genes. These mutations are lethal when combined with sec4-8 at 25 degrees C. Mutations that block elsewhere on the pathway are not suppressed by the SEC4 duplication and are not lethal when combined with sec4-8. We propose that the SEC4 product is a GTP-binding protein that plays an essential role in controlling a late stage of the secretory pathway.
PMID: 3552249 [PubMed - indexed for MEDLINE]
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Cited by over 100 PubMed Central articles
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The yeast LATS/Ndr kinase Cbk1 regulates growth via Golgi-dependent glycosylation and secretion.
Kurischko C, Kuravi VK, Wannissorn N, Nazarov PA, Husain M, Zhang C, Shokat KM, McCaffery JM, Luca FC.
Mol Biol Cell. 2008 Dec; 19(12):5559-78. Epub 2008 Oct 8.
[Mol Biol Cell. 2008]
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Swf1p, a member of the DHHC-CRD family of palmitoyltransferases, regulates the actin cytoskeleton and polarized secretion independently of its DHHC motif.
Dighe SA, Kozminski KG.
Mol Biol Cell. 2008 Oct; 19(10):4454-68. Epub 2008 Aug 13.
[Mol Biol Cell. 2008]
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Annexin A2 interactions with Rab14 in alveolar type II cells.
Gou D, Mishra A, Weng T, Su L, Chintagari NR, Wang Z, Zhang H, Gao L, Wang P, Stricker HM, et al.
J Biol Chem. 2008 May 9; 283(19):13156-64. Epub 2008 Mar 10.
[J Biol Chem. 2008]
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