A new lux gene in bioluminescent bacteria codes for a protein homologous to the bacterial luciferase subunits.

Department of Biochemistry, McGill University, Montreal, Quebec, Canada.

The nucleotide sequence of a new gene, luxF, located between the luxB and E genes in the bioluminescent system of Photobacterium phosphoreum has been determined. The luxF gene codes for a polypeptide of 231 amino acids which is homologous to the alpha and beta subunits of luciferase coded by the luxA and luxB genes, respectively. The degree of homology of the luxF protein is very high with the beta subunit of luciferase (approximately 30% identity) with greatest similarity to the Vibrio luxB proteins. the luxF gene appears to have evolved by duplication of the luxB gene followed by deletion of approximately 100 codons just penultimate to the 5'-terminal. The close homology with the luciferase beta subunit implicates the luxF protein in a function related to the light-emitting reaction.

PMID: 3415691 [PubMed - indexed for MEDLINE]