Isolation and characterization of the c-fos(rat) c...[Oncogene. 1987]

SearchDatabase nameforSearch term

Advanced Search

Your browser version may not work well with NCBI's Web applications. More information here...

Display Show

All: 1

Review: 0

Click to change filter selection through MyNCBI.

1: Oncogene. 1987;2(1):79-84.Links

Isolation and characterization of the c-fos(rat) cDNA and analysis of post-translational modification in vitro.

Curran T, Gordon MB, Rubino KL, Sambucetti LC.

Department of Molecular Oncology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.

c-fos mRNA accumulates to a level of 0.2% of cellular poly(A)-containing RNA 45 min after treatment of rat pheochromocytoma (PC12) cells with 1 mM barium chloride. Several clones of the c-fos(rat) cDNA were isolated from a cDNA library constructed from this RNA population. Nucleotide sequence analysis of a full-length cDNA clone reveals striking conservation among the c-fos genes isolated from rat, mouse and human cells, and confirms the c-fos gene structure predicted from an analysis of c-fos genomic clones. Translation of an SP6-derived transcript of the c-fos(rat) cDNA in a messenger-dependent rabbit reticulocyte lysate yields the complete c-fos protein. It undergoes extensive post-translational modification in the lysate, particularly in the presence of additional cAMP. The c-fos protein synthesized in vitro appears to be phosphorylated by the cAMP-dependent protein kinase.

PMID: 3325886 [PubMed - indexed for MEDLINE]

Isolation and structural analysis of a biologically active chicken c-fos cDNA: identification of evolutionarily conserved domains in fos protein.
Oncogene. 1987; 1(4):377-85.

[Oncogene. 1987]
Barium modulates c-fos expression and post-translational modification.
Proc Natl Acad Sci U S A. 1986 Nov; 83(22):8521-4.

[Proc Natl Acad Sci U S A. 1986]
Characterization and chromosomal localization of the human proto-oncogene BMI-1.
Hum Mol Genet. 1993 Oct; 2(10):1597-603.

[Hum Mol Genet. 1993]
Isolation and developmental expression of a rat cDNA encoding a cysteine-rich zinc finger protein.
Nucleic Acids Res. 1994 Dec 11; 22(24):5477-83.

[Nucleic Acids Res. 1994]
Isolation and characterization of a 14.5-kDa trichloroacetic-acid-soluble translational inhibitor protein from human monocytes that is upregulated upon cellular differentiation.
Eur J Biochem. 1996 Dec 1; 242(2):339-51.

[Eur J Biochem. 1996]
» See reviews... | » See all...

Cited by 48 PubMed Central articles

The dextromethorphan analog dimemorfan attenuates kainate-induced seizures via sigma1 receptor activation: comparison with the effects of dextromethorphan.
Shin EJ, Nah SY, Kim WK, Ko KH, Jhoo WK, Lim YK, Cha JY, Chen CF, Kim HC. Br J Pharmacol. 2005 Apr; 144(7):908-18.

[Br J Pharmacol. 2005]
ReviewIschemia-reperfusion-related repair deficit after oxidative stress: implications of faulty transcripts in neuronal sensitivity after brain injury.
Liu PK. J Biomed Sci. 2003 Jan-Feb; 10(1):4-13.

[J Biomed Sci. 2003]
Transcriptional induction of MKP-1 in response to stress is associated with histone H3 phosphorylation-acetylation.
Li J, Gorospe M, Hutter D, Barnes J, Keyse SM, Liu Y. Mol Cell Biol. 2001 Dec; 21(23):8213-24.

[Mol Cell Biol. 2001]
» See all...

Recent Activity

Clear Turn Off Turn On

Isolation and characterization of the c-fos(rat) cDNA and analysis of post-translational m...Isolation and characterization of the c-fos(rat) cDNA and analysis of post-translational modification in vitro.

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

Display Show

Isolation and characterization of the c-fos(rat) cDNA and analysis of post-translational modification in vitro.

Related articles

Cited by 48 PubMed Central articles

Recent Activity