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Cloning and gene expression of Schistosoma mansoni protease.
Schistosomes utilize proteases (termed hemoglobinases) for degradation of host globin. cDNA clones encoding Schistosoma mansoni protease were isolated by immunologically screening an expression cDNA library with antisera raised against purified hemoglobinase. Confirmation of the identities of the clones was obtained immunologically and biochemically. The bacterially produced fusion protein encoded by one clone, lambda Hb2, degraded hemoglobin in vitro. The sequence of this clone suggested that this S. mansoni protease is synthesized in a precursor form in vivo. Gene titrations indicated that S. mansoni contains multiple genes corresponding to this cDNA. The expression of these genes may be regulated during the organism's life cycle since adult, female worms contained the highest abundances of homologous mRNA and protein compared to other stages.
PMID: 3305515 [PubMed - indexed for MEDLINE]
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Cited by 7 PubMed Central articles
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Cystatin capture enzyme-linked immunosorbent assay for serodiagnosis of human clonorchiasis and profile of captured antigenic protein of Clonorchis sinensis.
Kim TY, Kang SY, Park SH, Sukontason K, Sukontason K, Hong SJ.
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[Clin Diagn Lab Immunol. 2001]
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Yeast Gpi8p is essential for GPI anchor attachment onto proteins.
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[EMBO J. 1996]
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[Infect Immun. 1996]
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