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Isolation of the Candida tropicalis gene for P450 lanosterol demethylase and its expression in Saccharomyces cerevisiae.
We have isolated the gene for cytochrome P450 lanosterol 14 alpha-demethylase (14DM) from the yeast Candida tropicalis. This was accomplished by screening genomic libraries of strain ATCC750 in E. coli using a DNA fragment containing the yeast Saccharomyces cerevisiae 14DM gene. Identity of this gene was confirmed by a) observing a heme binding region common to all P450s after sequencing the 3' portion of the gene, and b) based upon tests of its expression in strains of Saccharomyces cerevisiae.
PMID: 3304292 [PubMed - indexed for MEDLINE]
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Cited by 7 PubMed Central articles
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Experimental induction of fluconazole resistance in Candida tropicalis ATCC 750.
Barchiesi F, Calabrese D, Sanglard D, Falconi Di Francesco L, Caselli F, Giannini D, Giacometti A, Gavaudan S, Scalise G.
Antimicrob Agents Chemother. 2000 Jun; 44(6):1578-84.
[Antimicrob Agents Chemother. 2000]
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ReviewClinical, cellular, and molecular factors that contribute to antifungal drug resistance.
White TC, Marr KA, Bowden RA.
Clin Microbiol Rev. 1998 Apr; 11(2):382-402.
[Clin Microbiol Rev. 1998]
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Molecular biological characterization of an azole-resistant Candida glabrata isolate.
Marichal P, Vanden Bossche H, Odds FC, Nobels G, Warnock DW, Timmerman V, Van Broeckhoven C, Fay S, Mose-Larsen P.
Antimicrob Agents Chemother. 1997 Oct; 41(10):2229-37.
[Antimicrob Agents Chemother. 1997]
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