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Cloning, nucleotide sequence and characterization of genes encoding naphthalene dioxygenase of Pseudomonas putida strain NCIB9816.
Department of Genetics, University of Helsinki, Finland.
We have cloned the naphthalene dioxygenase(ND)-coding genes from Pseudomonas putida strain NCIB9816 based on their ability to convert indole to indigo. The region coding for this enzyme activity was sequenced and three successive open reading frames were found. The corresponding gene products were identified using the T7 polymerase/promoter system. All of them are necessary for the ND activity. A comparison of the ND-coding genes with the ones coding for benzene dioxygenase revealed significant homology which was more pronounced at the nucleotide level than at the amino acid level.
PMID: 3243438 [PubMed - indexed for MEDLINE]
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Cited by 48 PubMed Central articles
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Two angular dioxygenases contribute to the metabolic versatility of dibenzofuran-degrading Rhodococcus sp. strain HA01.
Aly HA, Huu NB, Wray V, Junca H, Pieper DH.
Appl Environ Microbiol. 2008 Jun; 74(12):3812-22. Epub 2008 Apr 25.
[Appl Environ Microbiol. 2008]
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Molecular cloning and expression of genes encoding a novel dioxygenase involved in low- and high-molecular-weight polycyclic aromatic hydrocarbon degradation in Mycobacterium vanbaalenii PYR-1.
Kim SJ, Kweon O, Freeman JP, Jones RC, Adjei MD, Jhoo JW, Edmondson RD, Cerniglia CE.
Appl Environ Microbiol. 2006 Feb; 72(2):1045-54.
[Appl Environ Microbiol. 2006]
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5-Hydroxyquinoline-2-Carboxylic Acid, a Dead-End Metabolite from the Bacterial Oxidation of 5-Aminonaphthalene-2-Sulfonic Acid.
Nörtemann B, Glässer A, Machinek R, Remberg G, Knackmuss HJ.
Appl Environ Microbiol. 1993 Jun; 59(6):1898-1903.
[Appl Environ Microbiol. 1993]
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